Masingano a jakisoni wa chromatograph wa gasinthawi zambiri amagwiritsa ntchito 1ul ndi 10ul. Ngakhale singano ya jakisoni ndi yaying'ono, ndiyofunikira. Singano ya jekeseni ndi njira yolumikizira chitsanzo ndi chida chowunikira. Ndi singano ya jakisoni, chitsanzocho chimatha kulowa mugawo la chromatographic ndikudutsa chowunikira kuti chiwunikidwe mosalekeza. Chifukwa chake, kukonza ndi kuyeretsa singano ya jekeseni ndiye chidwi cha akatswiri tsiku lililonse. Apo ayi, sizidzangokhudza kugwira ntchito bwino, komanso kuwononga chidacho. Chithunzi chotsatirachi chikuwonetsa zigawo za singano ya jekeseni.
Gulu la singano za jakisoni
Malinga ndi maonekedwe a singano jakisoni, akhoza kugawidwa mu singano conical jekeseni singano, bevel singano singano, ndi lathyathyathya-mutu singano. Singano za conical zimagwiritsidwa ntchito popanga jakisoni wa septum, zomwe zimatha kuchepetsa kuwonongeka kwa septum ndikupirira jakisoni angapo. Amagwiritsidwa ntchito makamaka mu majekeseni odziwikiratu; singano za bevel zitha kugwiritsidwa ntchito pa jakisoni septa, yomwe ndi yosavuta kugwiritsa ntchito. Pakati pawo, singano za 26s-22 ndizoyenera kwambiri kugwiritsidwa ntchito pa jekeseni septa mu chromatography ya gasi; singano zojambulira mutu wathyathyathya zimagwiritsidwa ntchito kwambiri pa mavavu a jakisoni ndi ma pipette a zitsanzo zamachromatograph amadzi ochita bwino kwambiri.
Malinga ndi njira ya jakisoni, imatha kugawidwa kukhala singano ya jakisoni yokhayo komanso singano ya jakisoni.
Malinga ndi kuwunika kosiyanasiyana kwa singano ya jakisoni mu chromatograph ya mpweya ndi madzi a chromatograph, imatha kugawidwa kukhala singano ya jekeseni wa gasi ndi singano ya jakisoni wamadzi. Sino ya jakisoni wa mpweya wa chromatography nthawi zambiri imafuna jekeseni wocheperako, ndipo voliyumu yodziwika bwino ya jakisoni ndi 0.2-1ul, motero singano yofananirayo nthawi zambiri imakhala 10-25ul. Singano yosankhidwa ndi singano yamtundu wa cone, yomwe ndi yabwino kwa jekeseni; Poyerekeza, voliyumu ya jakisoni yamadzimadzi ya chromatography nthawi zambiri imakhala yokulirapo, ndipo kuchuluka kwa jekeseni wamba ndi 0.5-20ul, kotero kuchuluka kwa singano wachibale kumakhalanso kokulirapo, nthawi zambiri 25-100UL, ndipo nsonga ya singano imakhala yosalala kuti mupewe kukanda stator.
Pakuwunika kwa chromatographic, singano yojambulira yomwe imagwiritsidwa ntchito kwambiri ndi singano yojambulira yaying'ono, yomwe ndiyoyenera kwambiri kusanthula kwamadzi kwa chromatograph yamafuta ndi madzi a chromatograph. Kulakwitsa kwake kwathunthu ndi ± 5%. Kuchita kwa mpweya kumapirira 0.2Mpa. Imagawidwa m'mitundu iwiri: jekeseni wamadzimadzi osungira madzi ndi jekeseni wamadzimadzi. Mafotokozedwe osiyanasiyana osakhala amadzimadzi jekeseni yaying'ono ndi 0.5μL-5μL, ndipo mawonekedwe amadzimadzi ang'onoang'ono jekeseni ndi 10μL-100μL. Singano yojambulira yaying'ono ndi chida chofunikira kwambiri.
Kugwiritsa ntchito jekeseni
(1) Yang'anani jekeseni musanagwiritse ntchito, fufuzani ngati syringe ili ndi ming'alu komanso ngati nsonga ya singano yapsa.
(2) Chotsani chitsanzo chotsalira mu jekeseni, sambani jekeseni ndi zosungunulira 5 ~ 20 nthawi, ndikutaya madzi otayika kuyambira nthawi 2 ~ 3.
(3) Chotsani thovu mu jekeseni, kumiza singano mu zosungunulira, ndipo mobwerezabwereza kujambula chitsanzo. Pamene kukhetsa chitsanzo, thovu mu jekeseni akhoza kusintha ndi kusintha ofukula chubu.
(4) Mukamagwiritsa ntchito jekeseni, choyamba mudzaze jekeseniyo ndi madzi, ndiyeno mukhetse madziwo mpaka mlingo wofunikira.
Kusamalira singano ya jekeseni
(1) Sing'anga ndi mkulu mamasukidwe akayendedwe zitsanzo ayenera kuchepetsedwa kapena lalikulu m'mimba mwake jekeseni singano ayenera kusankhidwa musanagwiritse ntchito.
(2) Poyeretsa singanoyo, payenera kugwiritsidwa ntchito zida zoyeretsera, monga waya wolondolera kapena masitayelo, zomangira, ndi zomangira zitsulo ziyenera kugwiritsidwa ntchito kuyeretsa khoma la singano.
(3) Kuyeretsa kwamafuta: Kutsuka kwamafuta kumagwiritsidwa ntchito kuchotsa zotsalira za organic pa singano, makamaka pofufuza, kuwira kwambiri komanso zinthu zomata. Pambuyo pa mphindi zochepa za kuyeretsa kwa kutentha, chida chotsuka singano chingagwiritsidwe ntchito kachiwiri.
Kuyeretsa singano ya jekeseni
1. Khoma lamkati la singano la jekeseni likhoza kutsukidwa ndi zosungunulira za organic. Mukamatsuka, chonde onani ngati jekeseni wokankhira singano akhoza kuyenda bwino;
2. Ngati jekeseni kankhira ndodo sikuyenda bwino, ndodo yokankhira ikhoza kuchotsedwa. Ndibwino kuti mupukuta ndi nsalu yofewa yoviikidwa mu zosungunulira za organic.
3. Gwiritsani ntchito organic zosungunulira mobwerezabwereza kuti aspirate. Ngati kukana kwa jekeseni singano kukankhira ndodo kumawonjezeka mofulumira pambuyo pa zokhumba zingapo, zikutanthauza kuti pali dothi laling'ono. Pankhaniyi, ntchito yoyeretsa iyenera kubwerezedwa.
4. Ngati singano yokankhira singano imatha kuyenda bwino komanso mosasunthika, fufuzani ngati singanoyo yatsekedwa. Muzitsuka singano mobwerezabwereza ndi zosungunulira za organic ndikuwona mawonekedwe a chitsanzo chomwe chikukankhidwira kunja kwa singano.
5. Ngati singano ya jakisoni ili yabwinobwino, chitsanzocho chimatuluka molunjika. Ngati singano yatsekeka, chitsanzocho chizipopera mu nkhungu yabwino kuchokera mbali imodzi kapena ngodya. Ngakhale ngati zosungunulira nthawi zina zimatuluka molunjika, samalani kuti muwone ngati kutuluka kwake kuli bwino kusiyana ndi nthawi zonse (ingoyerekezerani kuyenda ndi singano yatsopano, yosatsekedwa).
6. Kutsekeka mu singano kudzawononga kuberekanso kwa kusanthula. Pachifukwa ichi, kukonza singano ndikofunikira. Gwiritsani ntchito chinthu ngati waya kuti muchotse kutsekeka kwa singano. Singano ingagwiritsidwe ntchito ngati chitsanzo chikuyenda bwino. Kugwiritsira ntchito pipette kutsekemera madzi kapena chotsukira syringe kungathenso kuchotsa zonyansa mu singano.
Kusamala mukamagwiritsa ntchito singano ya jekeseni
Osagwira singano ya syringe ndi gawo lachitsanzo ndi manja anu, ndipo musakhale ndi thovu (pamene mukufuna, tsitsani pang'onopang'ono, mwamsanga, ndiyeno yesetsani pang'onopang'ono, bwerezani kangapo, 10 Voliyumu ya singano yachitsulo ya μl syringe ndi 0,6 μl Ngati pali thovu, simungawawone 1-2μl yochulukirapo ndikuloza nsonga ya singano mmwamba mpaka thovu likupita pamwamba kukankhira ndodo ya singano kuti muchotse thovu (Ponena za syringe ya 10μl, syringe yokhala ndi pachimake imakhala yosalala) Liwiro la jekeseni liyenera kukhala lofulumira (koma osati mofulumira), sungani liwiro lomwelo pa jekeseni iliyonse, ndikuyamba kubaya chitsanzo. pamene singano nsonga kufika pakati pa vaporization chipinda.
Mungateteze bwanji singano ya jakisoni kuti isapindike? Akatswiri ambiri omwe amasanthula chromatography nthawi zambiri amapinda singano ndi ndodo ya syringe ya syringe. Zifukwa zake ndi:
1. Khomo la jakisoni limakhomedwa mwamphamvu kwambiri. Ngati chatsekedwa mwamphamvu kwambiri kutentha kwa chipinda, chisindikizo cha silikoni chidzakula ndikumangitsa pamene kutentha kwa chipinda cha vaporization kukwera. Panthawiyi, zimakhala zovuta kuyika syringe.
2. Singano imayikidwa mu gawo lachitsulo la doko la jekeseni pamene malo sakupezeka bwino.
3. Ndodo ya syringe imapindika chifukwa mphamvu zambiri zimagwiritsidwa ntchito pobaya. Ma chromatograph odabwitsa, ochokera kunja amabwera ndi choyikapo jekeseni, ndipo kubaya ndi jekeseni sikungapindika ndodo ya syringe.
4. Chifukwa khoma lamkati la syringe ndi loipitsidwa, ndodo ya singano imakankhidwa ndikupindika panthawi yobaya. Mukatha kugwiritsa ntchito syringe kwa nthawi ndithu, mudzapeza chinthu chaching'ono chakuda pafupi ndi chubu cha singano, ndipo zidzakhala zovuta kuyamwa chitsanzo ndikubaya. Njira yoyeretsera: Tulutsani singano, bayani madzi pang'ono, ikani ndodo ya singano pamalo omwe ali ndi kachilombo ndikukankha ndikukoka mobwerezabwereza. Ngati sichigwira ntchito kamodzi, bayaninso madzi mpaka choipitsacho chichotsedwe. Panthawiyi, mudzawona kuti madzi a mu syringe ayamba chipwirikiti. Chotsani singano ndodo ndikupukuta ndi fyuluta pepala, ndiyeno kusamba ndi mowa kangapo. Pamene chitsanzo chiyenera kufufuzidwa ndi chitsanzo cholimba chosungunuka mu zosungunulira, sambani syringe ndi zosungunulira mu nthawi mutatha jekeseni.
5. Onetsetsani kuti mukukhazikika pobaya jakisoni. Ngati mukufunitsitsa kufulumizitsa, syringe imapindika. Malingana ngati mudziwa jekeseni, zidzakhala mofulumira.
Nthawi yotumiza: Jun-19-2024