Chonde pangani chisankho chanu kutengera momwe muliri mu labotale yanu.
Ndikofunikira kupeza njira yabwino yoyeretsera mbale zachitsanzo
Pakalipano, pali zitsanzo zambiri zaulimi (mankhwala ena, ma organic acid, ndi zina zotero) zomwe ziyenera kuyesedwa ndi madzi chromatography ndi gas chromatography chaka chilichonse.Chifukwa cha kuchuluka kwa zitsanzo, pali zitsanzo zambiri za mbale zomwe zimayenera kutsukidwa panthawi yodziwikiratu, zomwe sizimangowononga nthawi komanso zimachepetsa kugwira ntchito bwino, komanso nthawi zina zimayambitsa zopotoka pazotsatira zoyesera chifukwa cha ukhondo wa zotsukidwa zitsanzo mbale.
Mbale zachitsanzo za chromatographic zimapangidwa makamaka ndi galasi, nthawi zambiri zimakhala zapulasitiki.Zitsanzo za Mbale zotayidwa ndizokwera mtengo, zowononga, komanso zimawononga chilengedwe.Ma laboratories ambiri amatsuka mbale zachitsanzozo ndikuzigwiritsanso ntchito.
Pakalipano, njira zomwe zimagwiritsidwa ntchito m'ma labotale zimatsuka Mbale zimawonjezeredwa ndi zotsukira, zotsukira, zosungunulira za organic ndi kutsuka kwa asidi, kenako kutsukira kachubu kakang'ono.
Njira yoyeretsera iyi ili ndi zovuta zambiri:
Kugwiritsa ntchito zotsukira kumawononga madzi kwambiri, nthawi yotsuka ndi yayitali, ndipo pali ngodya zomwe sizingayeretsedwe.Ngati ndi pulasitiki chitsanzo Mbale, n'zosavuta kukhala ndi burashi zizindikiro mkati Mbale khoma, amene amatenga zambiri ntchito chuma.Kwa magalasi omwe amadetsedwa kwambiri ndi zotsalira za lipid ndi mapuloteni, njira ya alkaline lysis imagwiritsidwa ntchito poyeretsa, ndipo zotsatira zabwino zimapindula.
Pofufuza zitsanzo ndi LC/MS/MS, kuyeretsa mbale za jakisoni ndikofunikira kwambiri.Malinga ndi njira yoyeretsera magalasi, njira yoyeretsera imasankhidwa molingana ndi kuchuluka kwa kuipitsa.Palibe mawonekedwe okhazikika.Chidule cha njira:
Njira Yoyamba:
1. Thirani njira yoyesera muzitsulo zowuma
2. Dikirani njira zonse zoyesera mu 95% mowa, sambani kawiri ndi akupanga ndi kutsanulira, chifukwa mowa umalowa mosavuta mu 1.5mL vial ndipo ukhoza kusokonezeka ndi zosungunulira zambiri za organic kuti mukwaniritse kuyeretsa.
3. Thirani madzi oyera, ndi ultrasonically kusamba kawiri.
4. Thirani mafuta odzola mu mbale zouma ndikuphika pa madigiri 110 Celsius kwa maola 1 mpaka 2.Osaphika pa kutentha kwakukulu.
5. Kuzizira ndi kusunga.
Njira yachiwiri:
1. Muzimutsuka ndi madzi apampopi kangapo
2. Ikani mu beaker wodzazidwa ndi madzi oyera (Millipore koyera madzi makina) ndi sonicate kwa mphindi 15
3. Sinthani madzi ndi ultrasound kwa mphindi khumi ndi zisanu
4. Zilowerereni mu beaker wodzazidwa ndi ethanol mtheradi (Sinopharm Group, Analytical Pure)
5. Pomaliza, itulutseni ndikusiya kuti iume.
Njira yachitatu:
1. Choyamba zilowerere mu methanol (chromatographically woyera), ndi ultrasonically woyera kwa mphindi 20, ndiye kutsanulira methanol youma.
2. Lembani chitsanzo Mbale ndi madzi, ndi ultrasonically woyera kwa mphindi 20, kutsanulira madzi .
3. Yanikani mbale zachitsanzo pambuyo pake.
Njira Yachinayi:
Kusamba njira chitsanzo Mbale ndi chimodzimodzi ndi yokonza madzi gawo, etc. Choyamba, ntchito mankhwala mowa zilowerere kwa maola oposa 4, ndiye ultrasound kwa theka la ola, ndiye kutsanulira mankhwala mowa, ndi ntchito madzi. kwa theka la ultrasound.Maola, muzimutsuka ndi madzi ndikuwumitsa.
Njira 5:
Choyamba, zilowerereni amphamvu oxidizing kuyeretsa njira (potaziyamu dichromate) kwa maola 24, ndiyeno ntchito deionized madzi akupanga Sambani katatu pansi pa mikhalidwe, ndipo potsiriza kusamba ndi methanol kamodzi, ndiyeno ziume izo ntchito.
The caps septas ayenera kusinthidwa, makamaka popenda zotsalira za mankhwala ophera tizilombo, apo ayi zidzakhudza zotsatira za kuchuluka kwake.
Koma ngati zinthu zilola, yesani kugwiritsa ntchito zotayidwa, monga zotayidwa za PTFE kapena zoikamo zapulasitiki zapakhomo (pafupifupi 0.1 yuan/chidutswa), ndi mbale zachitsanzo zili bwino.Kugwiritsiridwa ntchito mobwerezabwereza ndipo sikuyenera kutsukidwa.
Njira 6:
(1) kusokoneza kuyeretsa ndi zotsatira zothandiza:
No1.Mbale zachitsanzo zitagwiritsidwa ntchito, tsukani mbale zachitsanzo ndi madzi othamanga poyamba, ndikutsuka chitsanzo chotsalira (mukhoza kuchigwedeza ndi dzanja nthawi yomweyo);
No2, kenaka ikani mbale zachitsanzo mu potassium dichromate kutsuka madzi kuwira, ndipo pamene achuluka Mukafika pamlingo winawake kapena mukakhala ndi maganizo abwino, chotsani mu thanki lodzola ndikuchiyika mu sieve ya pulasitiki kukhitchini. ntchito.Muzimutsuka bwino ndi madzi apampopi.Mutha kusefa mobwerezabwereza ndikugwedeza pakati;
No3.Gwiritsani ntchito madzi apampopi kuti muyeretse ultrasonically katatu mutatha kutsuka.Pozungulira, ndi bwino kugwedeza madzi mu chitsanzo Mbale pambuyo aliyense akupanga kuyeretsa;
No4, ndiye ntchito patatu distilled madzi (kapena oyeretsedwa madzi, deionized madzi) ndi 1.3 akupanga kuyeretsa katatu;
No5, ndiye ntchito chromatographic koyera methanol akupanga kuyeretsa 2-3 zina, ndi bwino kuti.
gwedezani methanol kuchokera ku mbale zachitsanzo pambuyo poyeretsa;
No6.Ikani mbale zachitsanzo mu uvuni ndikuwumitsa pafupifupi madigiri 80, ndipo zingagwiritsidwe ntchito.
(2) Mbale zitsanzo zogulidwa kuti mulembe ndi mitundu yosiyanasiyana:
ngati mwawona kuti pali kachidutswa kakang'ono pamiyendo yachitsanzo, yomwe siili yowoneka bwino, koma ili ndi ntchito yake.Pogula, ndi bwino kugula mbale zingapo zamitundu yosiyanasiyana.
Mwachitsanzo: labotale yanu imatsegula ntchito ziwiri A ndi B nthawi imodzi.Nthawi yoyamba A pulojekiti imagwiritsa ntchito mbale zoyera, ndipo polojekiti ya B imagwiritsa ntchito mbale za buluu.Mayeso akamaliza, amatsukidwa molingana ndi njira yomwe ili pamwambapa, ndipo kuyesera kwachiwiri Panthawiyo, gwiritsani ntchito mbale za buluu zachitsanzo za A pulojekiti, mbale zoyera zachitsanzo za polojekiti ya B, ndi zina zotero, zomwe zingathe kupeŵa bwino vuto lomwe limayambitsa kuipitsa ntchito yanu.
Lembani kumapeto
1. Akatswiri opanga zida zingapo anena kuti: Gwiritsani ntchito ng'anjo yamoto pa madigiri 400 kuphika kwa theka la ola, zinthu zakuthupi zapita;
2. Ikani mbale zachitsanzo mu ng'anjo ya muffle kuti ziume pa madigiri 300 Celsius.Katswiri wa Agilent wochokera ku Beijing adati atafika ku ng'anjo yamoto, mayesowo sadzakhala phokoso ataphika mu ng'anjo yamoto pa madigiri 300 kwa maola 6.
komanso………..
Mabotolo ang'onoang'ono a volumetric, ma flasks ooneka ngati mapeyala kuti azitha kuuluka mozungulira, ndi zida zina zamagalasi zowunikira kapena kuwongolera zitha kutsukidwa potengera njira iyi.
Nthawi yotumiza: Feb-25-2022