Gawo loyenda ndi lofanana ndi gawo lamadzimadzi lamagazi, ndipo pali zinthu zosiyanasiyana zomwe muyenera kuziganizira mukamagwiritsa ntchito. Pakati pawo pali "misampha" ina yomwe iyenera kutsatiridwa.
01. Yesani pH ya gawo la mafoni mutatha kuwonjezera zosungunulira za organic
Ngati muyeza pH ndi organic additive, pH yomwe mumapeza idzakhala yosiyana ndi yomwe isanawonjezerepo zosungunulira organic. Komabe, chinthu chofunika kwambiri ndicho kusasinthasintha. Ngati nthawi zonse mumayesa pH mutawonjezera zosungunulira za organic, onetsetsani kuti mwatchula njira zomwe mumagwiritsa ntchito kuti ena atsatire njira yomweyo. Njirayi si 100% yolondola, koma osachepera idzasunga njirayo. Izi zitha kukhala zofunika kwambiri kuposa kupeza pH yolondola.
02. Palibe buffer yomwe imagwiritsidwa ntchito
Cholinga cha buffer ndikuwongolera pH ndikuletsa kuti isasinthe. Njira zina zambiri zimasintha pH ya gawo la mafoni, zomwe zingayambitse kusintha kwa nthawi yosungira, mawonekedwe apamwamba, ndi kuyankha kwapamwamba.
Formic acid, TFA, ndi zina
03. Osagwiritsa ntchito buffer mkati mwa pH yanthawi zonse
Buffer iliyonse imakhala ndi 2 pH unit m'lifupi mwake, momwe imaperekera kukhazikika kwa pH kwabwino kwambiri. Ma buffers kunja kwa zenera ili sangapereke kukana koyenera kusintha kwa pH. Gwiritsani ntchito buffer munjira yoyenera, kapena sankhani bafa yomwe ili ndi pH yomwe mukufuna.
04. Onjezani zotchingira ku organic solution
Kusakaniza yankho la buffer ndi gawo lachilengedwe kungapangitse kuti buffer ifike. Nthawi zambiri, ngakhale kuti kwagwa mvula, zimakhala zovuta kuzizindikira. Kumbukirani kuti nthawi zonse muwonjezere yankho la organic pagawo lamadzi, lomwe lingachepetse kwambiri mwayi wa mvula.
05. Sakanizani gradient gradient kuchokera 0% ndi mpope
Mapampu omwe alipo lero amatha kusakaniza magawo am'manja ndi degas mkati, koma si onse omwe amagwiritsa ntchito njira yanu adzakhala ndi mpope wapamwamba kwambiri. Sakanizani A ndi B mu njira imodzi ndikuyendetsa 100% pamzere.
Mwachitsanzo, 950 ml ya organic kuyambira osakaniza akhoza kukonzekera kusakaniza ndi 50 ml ya madzi. Ubwino wa izi ndikuti ukhoza kuchepetsa kusiyana pakati pa ma HPLC ndikuchepetsa kuthekera kwa thovu ndi mvula mudongosolo. Ndikoyenera kudziwa kuti chiŵerengero cha chisakanizo cha mpope ndi 95: 5, zomwe sizikutanthauza kuti nthawi yosungirako yosakanizidwa mu botolo ndi 95: 5.
06. Osagwiritsa ntchito asidi wosinthidwa (base) kuti musinthe buffer
Gwiritsani ntchito asidi kapena maziko omwe amapanga mchere womwe mukugwiritsa ntchito. Mwachitsanzo, sodium phosphate buffer iyenera kukonzedwa ndi phosphoric acid kapena sodium hydroxide yokha.
07. Osanena zambiri za buffer mu njira, monga kuwonjezera 5g yasodium phosphate ku 1000ml madzi.
Mtundu wa buffer umatsimikizira mtundu wa pH womwe ungathe kusungidwa. Kuyika kofunikira kumatsimikizira mphamvu ya bafa. 5 magalamu kapena anhydrous sodium phosphate ndi 5 magalamu a monosodium phosphate monohydrate ali ndi mphamvu zosiyana za buffer.
08. Kuonjezera zosungunulira organic musanafufuze
Ngati njira yapitayi idagwiritsa ntchito njira yotchingira poyambira B, ndipo njira yanu imagwiritsa ntchito njira yachilengedwe yoyambira B, mutha kukhazikitsa chotchingira mu chubu la mpope ndi mutu wa mpope.
09. Kwezani botolo ndikukhuthula dontho lomaliza
Pali mwayi wabwino kuti simudzakhala ndi gawo lokwanira la mafoni kuti mumalize kuthamanga konse ndipo chitsanzo chanu chidzasuta. Kupatula kuthekera kowotcha makina opopera ndi gawo, gawo la mafoni lidzasungunuka kwathunthu ndipo gawo la mafoni pamwamba pa botolo lidzasintha.
10. Ntchito akupanga degassing mafoni gawo
Mfundo yofunika kwambiri ndikuwonetsetsa kuti mchere wonse wa bafa wasungunuka, koma iyi ndiyo njira yoyipa kwambiri yopangira degas ndipo imatenthetsa mwachangu gawo la mafoni, zomwe zimapangitsa kuti zida za organic zisunthike. Kuti muteteze vuto losafunikira pambuyo pake, tengani mphindi zisanu kuti musefe gawo lanu la foni yam'manja.
Nthawi yotumiza: Aug-27-2024